Product Name:
Mouse Mature Brain-derived Neurotrophic Factor (mBDNF) ELISA Kit
Cat.#:
JL37130
Regulatory Status:
Alternative:
Mature brain-derived neurotrophic factor
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.78-50ng/mL
Sensitivity:
0.35ng/mL
Specificity:
This kit recognizes Mouse mBDNF in samples. No significant cross-reactivity or interference between Mouse mBDNF and analogues was observed.
Sample Type:
Serum;plasma;tissue homogenates;cell lysates;cell culture supernates and other biological fluids
Assay Procedure:
Research Area:
Neuroscience
Size & Price: 
Product Datasheets
Instruction: 
| mBDNF
Brain-derived neurotrophic factor (BDNF), or abrineurin,[5] is a protein that is encoded by the BDNF gene. BDNF is a member of the neurotrophin family of growth factors, which are related to the canonical nerve growth factor (NGF), a family which also includes NT-3 and NT-4/NT-5. Neurotrophic factors are found in the brain and the periphery. BDNF was first isolated from a pig brain in 1982 by Yves-Alain Barde and Hans Thoenen.BDNF activates the TrkB tyrosine kinase receptor.
| Repeatability
Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%| Recovery
The recovery of Mouse mBDNF spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.| Sample Type | Range(%) | Average Recovery(%) |
| Serum(n=8) | 84-101 | 96 |
| Plasma(n=8) | 92-105 | 104 |
| Cell Culture Supernates(n=8) | 96-108 | 105 |
| Linearity
Samples were spiked with high concentrations of Mouse mBDNF and diluted with Universal Diluent to produce samples with values within the range of the assay.| Serum(n=4) | Plasma(n=4) | Cell Culture Supernates(n=4) | ||
| 1 : 2 | Range (%) | 84-95 | 88-96 | 90-110 |
| Average (%) | 91 | 93 | 96 | |
| 1 : 4 | Range (%) | 89-103 | 87-107 | 105-115 |
| Average (%) | 94 | 98 | 108 |
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